FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3264226
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG151310.4635402083066552No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC132500.40591552178066104No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC128120.3924973332116098No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN90610.27758494663053357No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT69250.21214830100611906No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC56110.1718937352989652No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG40160.12303069701668941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG29100.061.2149624
TCTACGG29000.060.9572645
TACGGCC29900.059.1224257
CTACGGC30950.057.1166576
CCGAACT10800.053.37571767
ACGGCCA33100.053.3033648
GCGCGGT68450.052.9998749
GCCATAC33250.052.7543911
CGGCCAT34100.051.7402159
GCCGGTT11100.051.440643
GGTCTAC28650.051.3164633
CCATACC34550.050.96578612
GGCCATA35250.050.0522410
GGGTCTA27050.049.8014342
CGCGGTG73200.049.60738810
CATACCA35650.049.297313
TGGCGCG74150.049.15627315
CGGTTCA12250.047.7301645
CCGGTTC12350.047.0646554
TACCACC38000.046.1600915