FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3434884
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG321160.9349951846991049No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA186180.5420270378854133No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC157890.4596661779553545No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC146900.4276709198913268No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG85440.2487420244759357No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN82420.23994987894787714No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT74620.2172416885111695No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG64390.18745902336148762No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA42900.12489504740189189No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG40940.11918888672805253No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA40360.11750032897763069No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC36460.10614623375927688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG60200.063.796564
TCTACGG60850.063.171245
TACGGCC61750.062.3058557
CTACGGC62150.061.9598276
GCGTCTA10350.059.419352
ACGGCCA65400.058.828548
CGGCCAT65650.058.7086149
GGGTCTA48550.057.9170382
CCTGGTT98000.057.43202670
GCCATAC67200.057.14024411
CCGAACT11250.056.77911467
CGTCTAC12600.056.1292463
CCATACC69250.055.55387512
GGCCATA69400.055.53631210
CATACCA69650.055.2348313
GCGCGGT80700.053.983889
CGGTCTA8100.053.569112
TACCACC72450.052.8164315
GCCGGTT11700.052.5625083
CGCGGTG83800.052.02763710