FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3413959
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG318070.9316749263831229No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA183220.5366789700755047No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC158440.4640946185938378No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC145890.42733377875949885No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG85910.2516433267066183No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN82080.24042467996833No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT75010.21971558533655502No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG62300.18248608140871053No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA43710.12803317204453832No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA40220.11781043650494925No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG39460.11558428206079803No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC36600.10720691138938693No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC35400.10369193068809554No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC34620.10140719323225617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG61050.064.118145
GTCTACG61250.063.908784
CTACGGC61650.063.4368556
TACGGCC61950.063.2931987
ACGGCCA65250.060.092178
GCGTCTA11650.058.6509932
CGGCCAT67250.058.254259
GGGTCTA48050.058.232012
GCCATAC67300.057.90646711
CCTGGTT98100.057.412670
CGTCTAC14400.056.9286273
GGCCATA69900.056.1923510
CATACCA69600.056.04196513
CCATACC70750.055.2758612
GCGCGGT75200.054.5937739
TACCACC72850.053.5886915
ATACCAC75150.051.90313714
CGGTCTA7850.051.7903182
CGCGGTG79550.051.60844410
CCGAACT11350.051.51233767
Produced by FastQC (version 0.11.5)