FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3434023
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG315060.9174661905293005No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA172870.5034037337548409No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC152400.4437943484944626No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC143190.41697449318190355No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG84760.2468242058949518No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN83710.2437665676671356No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT73270.21336490757342044No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG60760.17693533211629625No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA41470.12076214981670189No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG39640.11543312319107939No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA38020.11071562421102014No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC35930.10462946811946221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG60350.063.9006044
TACGGCC61100.063.18327
TCTACGG61150.063.0207185
CTACGGC62100.062.0566336
GGGTCTA46950.060.0372472
ACGGCCA65300.059.747168
CGGCCAT65550.059.310829
GCCATAC66950.057.91748411
CGTCTAC12050.057.8271063
CCTGGTT97100.057.06503370
CATACCA68800.056.20949613
CCATACC69000.056.19510712
GGCCATA69500.055.98908210
GCGTCTA11000.055.2878532
GCGCGGT83800.054.670019
TACCACC71200.054.1212915
CCGAACT12750.053.04606267
GCCGGTT12250.052.142743
CGCGGTG88400.052.05706810
ATACCAC74450.051.94224514