FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4416916
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC133480.30220180777719113No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC100210.22687775814618164No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA76300.17274496503895478No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC69780.15798353421255917No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT64930.14700302201807777No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46700.10572988030562501No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG45130.10217536398699908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT63400.050.875129
CCGAACT10300.047.89354367
TGGCGCG68700.047.35315315
CGCGGTG69650.046.40935510
GCCGGTT11700.042.7125173
CGGCAAT4800.042.5227431
CGGCATA3850.042.2325821
CGGTTCA12000.042.2138525
CTACTCG82150.039.97120737
GTAGTCC82750.039.89834627
CGTGCCT82800.039.81726520
TACTCGG82000.039.80741538
TCTACGG6850.039.7425235
TAGTCCC83700.039.4190128
TGTAGTC83750.039.33321826
CCGGTTC13000.038.7015534
CGAACTT13200.038.62699568
GTCTACG7100.037.8577774
ACTCGGG88100.037.7767139
CCTGTAG88550.037.33968724