FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4538475
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG286160.6305201637113788No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG117620.2591619431637279No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG76530.16862492357014194No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC59440.13096910305774515No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG49830.10979458959231901No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC49140.10827425511873481No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC48160.10611493948958627No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA47120.10382342086273473No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46780.10307427054241788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA83800.039.399332
GCGGCAC87150.037.89949433
CGGCACC88500.037.29629534
TCGCCGG87550.035.8467448
CCGTCGC87950.035.4976345
CCCGTCG89100.035.28725444
GCACCCC96150.034.64892236
GGCACCC98250.033.68317835
CCCCCGA99500.033.59045819
CACCCCC101200.033.04135537
ACCCGCG103300.032.25897225
GTGCCGT8800.031.3786471
CCCCCGT101000.030.85362242
CCCCGAC109450.030.44593620
AGGACCG11150.030.10406770
TATCGGA3350.029.841088
CGCCCTA18600.029.81744261
TAGTCCG6200.029.4676516
AGTCCGC119250.028.3288547
AGGAGGG155800.028.00760370