FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4496719
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG279700.6220090692791789No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG115220.25623126550713976No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG72080.16029465038842766No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC57610.12811563275356988No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG48760.10843461643923046No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC47500.10563257343854486No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA45930.10214113890594453No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45720.1016741317391636No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC45560.10131831675494955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA81350.037.79508632
GCGGCAC84250.036.6276533
CGGCACC84900.036.1974134
CCGTCGC83400.035.20447545
TCGCCGG83500.035.06408348
CCCGTCG84750.034.62617544
GCACCCC92000.033.5039736
AGGACCG10050.033.33011670
GGCACCC95950.032.07779335
CCCCCGA97100.032.04520819
CACCCCC97000.031.82945437
GTGCCGT7500.031.7789551
CGTACGT3500.031.53971119
ACCCGCG98900.031.2613425
CCCCCGT98700.029.73338542
CCCCGAC105200.029.55032220
CGATCTA3900.028.61756547
CGCCCTA18100.028.09120461
CGTCGCC107550.027.28852546
AGTCCGC115250.027.135277