FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4496719
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC140100.3115604955524239No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC104780.2330143377871733No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA79120.17595050969384565No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC70450.15666978523674707No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT64270.14292643147147954No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG51060.11354945683730738No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45690.10160741642962347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT65600.051.6409239
TGGCGCG69400.049.011415
CGCGGTG70100.048.4733710
CCGAACT11650.048.47310667
GCCGGTT12350.044.3679773
CGGTTCA12100.044.145435
CGAACTT13900.042.9516568
CTACTCG81950.041.59589837
TACTCGG82100.041.36299538
CGGCATA2950.041.057521
GTAGTCC83400.040.92654427
CGGCAAT5500.040.897561
CGTGCCT83800.040.7427120
TAGTCCC84850.040.4026428
CCGGTTC13550.040.184384
TGTAGTC85100.040.17985526
ACTCGGG88150.039.13060839
CCTGTAG87950.039.017524
GGCGCGT89000.038.48892616
GCGTGCC90550.038.04113419