FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3733660
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG424001.1356149194088374No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA228510.6120268047974373No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC199970.535587064703267No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN183140.4905106517465436No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC173600.46495931605984475No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG119390.31976666327410636No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT92120.2467284112640144No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG81160.2173738369321256No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG52950.1418179480724008No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA50150.13431860426498396No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC44780.11993593417718806No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA43440.1163469624979243No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA41010.1098386034079161No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC40610.10876726857828511No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC39730.10641033195309696No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC39440.10563361420161452No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC39390.10549969734791062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG85450.063.9473725
GTCTACG85850.063.6494264
TACGGCC86550.063.2127727
CTACGGC87300.062.7471736
GGGTCTA65150.060.581972
ACGGCCA90400.060.408438
CGGCCAT91950.059.573999
GCCATAC91300.059.54968311
GCGTCTA16500.058.6231352
CCATACC93350.058.3143912
GGCCATA94050.058.0601210
CATACCA94050.057.88036713
CCTGGTT137050.056.7334870
CGTCTAC19500.056.703833
TACCACC97400.055.61264815
ATACCAC100500.054.13201514
GCGCGGT104600.054.1149449
CGGTCTA9100.053.519012
CGCGGTG106150.053.4167210
ACCACCC102700.052.7426716
Produced by FastQC (version 0.11.5)