FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3128396
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA101450.32428759018998876No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC92890.2969253253104786No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC78220.2500322849153368No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN53600.17133380812403545No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT41060.1312493686860615No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC33870.10826634479778134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT43550.053.9833879
GTCTACG6450.051.080524
CGCGGTG46850.050.47395310
TGGCGCG47400.048.87067815
TCTACGG6850.048.0977135
CCGAACT6850.047.22341567
TACGGCC6950.046.9133577
ATCTCGT7000.045.476635
GCCGGTT7550.044.9998633
CTACTCG56050.042.2852837
GTAGTCC56450.041.9136427
TACTCGG57050.041.68375438
CGGGTAG4500.041.3851431
CGGTTCA8050.041.3542025
TGTAGTC57150.041.31941226
GGCGCGT56950.041.27810716
TAGTCCC57600.041.22157728
CGTGCCT57350.041.09281520
GGGGACT36550.040.856741
ACTCGGG59700.039.62555739
Produced by FastQC (version 0.11.5)