FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3115149
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA101450.3256666053533876No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC95080.3052181452636776No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC81860.26278036780905184No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51560.16551375231168716No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT42110.13517812470607346No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC34500.11074911665541519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT43350.050.857029
GTCTACG6050.049.9528434
TACGGCC6100.048.977257
TCTACGG6250.048.354355
TGGCGCG46600.047.45674515
CGCGGTG46800.047.4014510
CCGAACT7450.044.8788367
GCCGGTT7700.041.032693
GGGGACT36650.041.00461
CGGCATA2550.040.6441841
CTACGGC7450.040.5650836
CGGTTCA7900.040.4286085
CTACTCG55800.040.2186237
ACTCTTC32950.039.8144655
TACTCGG56550.039.71226538
CGTGCCT55850.039.70813820
TCTGGTC32950.039.49924510
GTAGTCC57000.039.2297627
GCGGTAT1850.039.2161451
GGGACTC36800.038.9260062
Produced by FastQC (version 0.11.5)