FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212162
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG24320.20063324869118154No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21530.17761652320399418No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC19110.15765219500363814No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC17150.1414827391058291No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG13670.11277370516482119No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13670.11277370516482119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA7350.040.0928232
TCGCCGG7750.037.3992448
ACCCGCG8000.037.15746725
CGTTGGA2350.036.89824333
CGGCACC8000.036.86284634
CCCGTCG8050.036.35862744
GCGGCAC8150.036.1738633
CCGTCGC8150.035.49683845
CCCCCGA8600.034.10119
CCCCGAC8850.033.1527820
CCGCGCG9250.032.5449227
CGGATAG652.3364037E-531.816614
TCGCGAT450.004256540431.18837757
AATCGCG450.004275847731.15954855
CCCCCGT9250.030.86267742
GCGACGA450.004646646330.6344095
TCGTTGG3000.030.04602832
TTCGTTG2900.029.88415131
TATTCGG703.875656E-529.54398310
GCGCGGG10050.029.43283362