FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212162
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21510.17745152875605735No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA17900.1476700309034601No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC17000.14024528074630288No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG15070.1243233165203991No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC14870.12267337204103082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG3250.056.163744
TCTACGG3300.055.312775
TACGGCC3300.055.3104867
CTACGGC3500.052.152046
CCGTATA750.050.8059231
GCGCGGT10950.047.797299
CGGTATA1400.047.0119741
TGGCGCG11300.046.62347815
GGCGCGT12050.044.8646516
CGGTAGA1400.044.537661
GAACGCG4150.044.10574324
GCGTATT1900.043.75631
CGCGTGC12400.043.63075618
CGCGGTG12250.042.72492210
GCGTGCC13000.041.63424319
GCGCATA758.385541E-1041.568481
GCGCGTG13150.041.38554417
CGGTAAT1850.041.1939931
GCGTACT1100.040.9386561
GGCGAAT1700.040.753411
Produced by FastQC (version 0.11.5)