FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207795
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21650.17925227377162514No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC16710.13835129305883861No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA16410.13586742783336575No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG16080.1331351760853456No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC15330.12692551302166344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG4650.060.746335
TACGGCC4950.057.7582477
CTACGGC4950.057.7582476
GTCTACG4900.057.6470264
GAACGCG5400.052.42022724
GCGCATA950.051.0933881
GCGCGGT10450.048.7850239
ATCTCGT5950.048.3760335
TGGCGCG10450.048.1237715
GCCCGAT6100.047.09991530
CGGTATA1400.047.0528371
CCCGATC6100.046.5537931
CGGTATG1500.046.2273521
CGTAATA605.68798E-945.930152
GCCATAC6350.045.56471611
TGAACGC6350.045.10458823
CTGAACG6350.045.08585422
GGCGCGT11100.044.99726516
CGGTATC851.8189894E-1244.867721
CGCGGTG11400.044.41744610
Produced by FastQC (version 0.11.5)