FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3630731
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG162720.4481742106479384No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG71920.1980868315499No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG47760.13154375799253648No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG44550.12270256320283711No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC38730.10667273339721393No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36570.10072351821162186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT18700.037.56997370
CGCGGCA51450.036.3198732
AGGACCG9750.034.9843370
GCGGCAC53750.034.71382533
CGGCACC55800.033.3245734
CCGTCGC52550.033.17917645
CCCGTCG53700.032.64579844
TCGCCGG52900.032.62030848
ATGGCCG18150.031.27084769
GTTCCGT5000.031.1386851
GCACCCC60400.030.9770436
CCCCCGA61050.030.84927619
CACCCCC64150.029.23157337
GACGTAT4450.028.92096344
CCCCCGT61350.028.49061642
ACCCGCG66550.028.17962325
GGCACCC66750.027.92015535
TACGGGT4850.027.7267656
CCCCGAC68450.027.57069820
CGATCTA2050.027.19447747