FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3630731
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG129450.3565397711920823No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC116410.32062413877535956No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC95680.26352819859141313No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT62850.17310563630299242No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC36840.10146717010982086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG27750.060.6177144
TACGGCC27850.060.0287367
TCTACGG28100.059.8626865
CTACGGC28550.059.1606146
ACGGCCA31300.053.6331528
GCCATAC31650.052.6051311
CGGCCAT32150.052.108679
GCGCGGT51800.051.1076749
CCGAACT9900.049.81920267
TGAACGC33650.049.3314523
GGGTCTA26500.048.9146042
CCTGGTT49700.048.71836570
GAACGCG34100.048.69053624
CCATACC34550.048.39052612
CATACCA34850.048.0728813
GCGTCTA6400.047.940952
TGGCGCG55400.047.78791415
GGTCTAC29150.047.6570473
GGCCATA35600.047.44612510
ATCTCGT34850.047.30023235
Produced by FastQC (version 0.11.5)