FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3624978
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG174790.4821822366921951No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG74390.20521503854644083No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG50650.1397249859171559No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG43510.12002831465459929No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC39450.10882824668177297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA51300.037.7761132
GCGGCAC54450.035.53799433
CGGCACC55350.035.16189234
CCGTCGC53550.034.84842345
ACGATCG601.3469089E-534.486656
CCCGTCG54500.034.48208244
TGGCCGT17650.034.26598470
TCGCCGG54650.033.96269248
TCGGATA5750.033.12442834
GCACCCC61100.031.98929236
CGTATCA6100.031.98399446
CCCCCGA61900.031.88792619
GACGTAT5950.031.58442944
AGGACCG10450.030.38441770
CCCCCGT61900.030.04188742
CGGATAG6350.030.00620835
CACCCCC65700.029.7087637
GGACGTA6500.029.42885443
GGCACCC66400.029.32178135
ATGGCCG16400.029.21783469
Produced by FastQC (version 0.11.5)