FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3624978
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG127450.3515883406740675No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC119020.3283330271245784No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC101100.2788982443479657No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT61870.17067689790117346No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG40190.11086963838125362No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC37200.10262131246037906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG25600.061.815614
TACGGCC26200.060.6631667
TCTACGG26500.059.846315
CTACGGC27750.057.3990146
ACGGCCA29650.053.6045538
GCGCGGT56350.052.5562869
GCCATAC30600.051.82554611
CCGAACT10500.051.03076667
CGGCCAT31750.050.1676459
TGGCGCG59600.049.80538615
CATACCA32100.049.2963913
CCATACC32150.049.21972312
GGGTCTA24050.048.3183632
CGCGGTG62000.048.0429310
CGGTTCA11150.047.9272235
GGTCTAC26150.047.858643
GCCGGTT11100.047.832513
GGCCATA33400.047.79054310
GCGTCTA6100.047.4839552
GAACGCG33750.047.15770324
Produced by FastQC (version 0.11.5)