FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3735387
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG177910.4762826448772242No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG76650.2051996218865676No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG49690.1330250386372282No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG45220.12105840706732662No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC41000.10976105019372825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG10300.038.72711670
CGCGGCA55550.037.01445432
GCGGCAC58900.034.97598333
CGGCACC59250.034.7796734
CCGTCGC57950.034.01901245
CCCGTCG58250.033.87975344
TCGCCGG58700.033.7007148
TGGCCGT17200.031.99803770
GCACCCC66250.031.29156936
CCCCCGA67000.030.88624619
CCCCCGT65050.030.24821742
CACCCCC69500.030.1438237
GGCACCC70400.029.3354435
ACCCGCG70700.029.30755825
CGGATAG5500.028.98105235
GACGTAT5550.028.22101644
CCCCGAC74150.027.77106920
TAGTCCG3850.027.7578566
CGTATCA5700.027.51818546
TCGGATA5800.027.46797834