FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3684010
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG174030.4723928545253678No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG74090.20111237483068722No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG50210.1362917038770253No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG44140.11981509279290774No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC39780.10798016292029608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG10450.038.2702970
TGGCCGT17850.034.31611370
CGCGGCA55950.034.14455832
GCGGCAC58150.032.86261733
CGGCACC59550.031.80890334
TCGCCGG58950.030.87510748
CCGTCGC59200.030.8641845
CCCGTCG59850.030.63141844
ATGGCCG16450.029.43994769
GACGTAT4850.029.40781644
GCACCCC66800.028.7909536
CCCCCGA67350.028.58725219
CACCCCC69050.027.91517437
CCCCCGT65900.027.52666942
GGCACCC70950.027.04588735
GGCGATC10400.026.77196570
GTGCCGT6600.026.6886961
GGACGTA5500.026.55247143
GTTCCGT4700.026.4548781
CGGATAG5400.026.2986835
Produced by FastQC (version 0.11.5)