FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3684010
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG124180.33707834669287No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC121060.32860931430696444No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC99910.2711990466909699No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT62580.1698692457403753No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG39930.10838732793884923No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC36900.10016259456407554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG26400.062.018194
TCTACGG27250.060.33835
TACGGCC27050.060.275527
CTACGGC28300.057.978596
GCCATAC31550.051.3499111
ACGGCCA32150.051.250028
GCGCGGT60300.051.2190939
CGGCCAT32850.049.7375229
GGGTCTA24150.048.6705932
TGGCGCG64500.048.04226315
GGCCATA34200.047.67341210
CCGAACT10800.047.61421667
GAACGCG34300.047.51203524
CCTGGTT50000.047.07089270
CGCGGTG65950.047.04017310
CCATACC34400.046.99415212
CATACCA34900.046.61654313
TGAACGC35000.046.54976323
GCGTCTA7000.046.2870182
ATCTCGT35250.045.87293235
Produced by FastQC (version 0.11.5)