FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3158789
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC237710.752535227899046No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG220700.6986854772509339No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC213810.6768733207567836No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA146220.4628989147423269No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT104950.33224757968955826No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG81170.2569655649680938No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN64290.20352736444251263No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC54020.171014904762553No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC49850.15781364313982352No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC48220.15265343775731777No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG47190.14939269447880185No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC45340.14353601965816648No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC41390.13103122747356663No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC40790.12913176536957677No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA33280.10535683136797046No Hit
GCGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC31860.10086143772186112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG50400.063.8679664
TCTACGG50400.063.79985
TACGGCC51250.063.0097857
CTACGGC51600.062.382666
ACGGCCA53650.060.1910828
CGGCCAT54500.059.5044569
GCGTCTA11750.058.7733462
GCCATAC55350.058.46652211
GGGTCTA36550.058.3749432
CGTCTAC14100.057.4998743
CGGTCTA6300.057.2625922
GGCCATA56700.057.13324710
GCCGGTT14100.056.7689443
CCTGGTT79450.056.6949470
CATACCA56950.056.52230513
CCATACC57300.056.3569212
CCGAACT14600.055.63899267
CGGTTCA14650.054.8721855
GCGCGGT106650.054.6309789
TACCACC58650.054.0639415
Produced by FastQC (version 0.11.5)