FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3137196
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC231670.7384619896238551No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG210320.6704075869024442No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC209870.6689731849715479No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA148600.47367139318040696No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT103390.32956181252303013No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG76960.24531460578172357No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN62590.19950937078843656No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC53120.16932317904268654No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC48250.15379976259054265No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC47220.15051657594871343No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG46160.14713776251149116No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC44840.14293018351419548No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC40320.12852241300830425No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC37920.12087226937685755No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA31860.10155565670745467No Hit
GCGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC31850.10152378110899031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG48050.062.8919334
TCTACGG47950.062.8857235
TACGGCC48200.062.7032247
CTACGGC49550.060.9948586
ACGGCCA50100.060.5311438
GCCATAC52300.057.26173411
CGGCCAT52750.057.2295159
GGGTCTA34450.057.182732
CCATACC53700.056.08812312
CATACCA53650.056.07541313
CGTCTAC13000.056.065023
GCGTCTA11600.055.7184872
GCGCGGT110550.055.5793959
CCGAACT16300.054.85310467
GGCCATA54950.054.75053410
CCTGGTT78000.054.32584470
CGGTTCA16500.054.178945
GCCGGTT16450.054.1294143
CGCGGTG114100.053.85015510
TACCACC56150.053.57958615
Produced by FastQC (version 0.11.5)