FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3235437
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC47490.14678079035382235No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC42750.1321305282717605No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC39120.12091102376587767No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG34410.10635348486155038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT26400.049.1628469
TGGCGCG28250.046.18985715
CGCGGTG31350.041.51232510
CTACTCG32550.039.56103537
TACTCGG32350.039.388738
CGGGTAG4350.039.0267071
CGGTAAG2700.038.4957281
GGGGTAG14500.037.99131
CGGCATA2800.037.1208841
ACTCGGG35800.037.06087539
GTAGTCC36350.036.44646527
TAGTCCC36450.036.26506428
CGGCTAG3000.035.801031
GGTCGTA1550.035.6326522
AGGATCG36300.035.35303559
TGTAGTC37500.035.3205626
TCGCGTA601.1733235E-535.1881560
CGGCAAA6250.034.3689881
CCTGTAG39750.033.82965524
CCGAACT4950.033.81950467
Produced by FastQC (version 0.11.5)