FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3235917
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG180080.556503766938398No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG84770.2619659280506886No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG49720.15365041810404903No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC43200.13350157003408927No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA39800.12299450202214704No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC39600.12237643919791515No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC36320.11224020888051207No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA34370.10621409634425111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA58250.035.20451732
GCGGCAC59150.034.67962633
CGGCACC61450.033.4494234
CCGTCGC59150.032.37050245
TCGCCGG59100.032.10604548
CCCGTCG60750.031.73034344
GCACCCC66850.030.87272336
CGCGCTA1151.6534614E-930.08682628
TACGGTC1151.6734703E-930.0537511
CCCCCGA69350.029.839319
CACCCCC69250.029.81573937
GGCACCC69950.029.49581735
CGTACGT2400.028.78902819
GGCGATC11750.027.96658170
GCGTATA756.131487E-527.6233585
ACCCGCG74850.027.44480725
CGCCCTA16600.027.38750361
GTAGCGT3900.026.5617410
AGTCCGC79800.026.3953157
CAGTCCG76450.026.3771936