FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3235917
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC48550.15003475058229243No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC45010.1390950385933879No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC40170.12413791824697606No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG37070.11455794447138168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA407.205126E-752.79438460
GCGCGGT25350.048.8924839
TGGCGCG27000.045.64887215
CTACTCG31200.040.7043837
TACTCGG31400.040.56760438
CCGAACT5650.040.3494167
CGGGTAG4650.040.2216031
CGCGGTG31150.039.8978910
GGGGTAG14550.039.0390661
TACGCAC4250.038.35061666
CGAACTT6600.037.9376868
GCCGGTT6050.037.6629373
GTACGCA4700.037.62320365
AGGATCG32650.037.5873459
ACTCGGG35250.037.4308439
CGGCAAT3900.037.2995221
GTAGTCC34300.036.50229327
TAGTCCC34600.036.2953228
AAGTACG5400.035.9472563
TGTAGTC35450.035.70232426
Produced by FastQC (version 0.11.5)