FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3330676
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG182100.5467358578258588No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG88120.26457091593418275No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG51260.15390269122544492No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC44810.13453725309816986No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA40850.12264777480607542No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC39440.11841439995964782No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC37430.11237958900835747No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA35290.10595446690101348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA59750.036.58325632
GCGGCAC62900.034.8162333
CGGCACC64950.033.73208634
TCGCCGG61600.033.63866848
CCCGTCG63300.033.0987544
CCGTCGC62650.033.07483745
TATCGGA2850.032.710328
GCACCCC69050.032.1492436
CCCCCGA71800.031.12637319
GTTATCG2900.030.9557426
CACCCCC72700.030.73619337
GGCACCC71800.030.66847835
ACCCGCG75450.029.51874525
TTATCGG3200.029.132637
ATCGGAA3200.028.0536449
CCCCGAC80450.027.91182120
CGCCCTA18700.027.50414761
AGGAGGG104050.027.21064470
AGTCCGC83900.026.6672947
CAGTCCG81350.026.6543436