FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3284667
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG178700.5440429730015249No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG85330.2597828029447125No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG49850.15176576499231126No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC42600.12969351231038032No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA40640.12372639296464451No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC39410.11998172113033073No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC36790.11200526567837774No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA33730.10268925282228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA56750.036.3271532
GCGGCAC59500.034.5417333
CGGCACC60050.034.23216634
CCGTCGC57100.033.7631545
TCGCCGG57650.033.49347748
CCCGTCG58650.033.21615644
GCACCCC64050.032.05539336
CCCCCGA67600.030.67171719
CGTACGT2750.030.15866319
CACCCCC68000.030.15665237
GGCACCC68950.029.81715835
CGATCTA2750.029.13295747
ACCCGCG70550.029.12149425
CGCCCTA16050.028.30924861
TCCGCTA1601.8189894E-1228.1194041
GTTCTCG22200.027.8523313
CTCGTTT22250.027.78973816
AGTCCGC76050.027.4363067
TCGTTTG22600.027.21066717
CCCCGAC76550.027.08938820