FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3284667
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC47390.14427642132368365No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC43650.13289018338845307No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC38570.11742438426787251No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG38550.11736349529495685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT25050.047.150079
TGGCGCG27950.041.63886315
CCGAACT5300.040.30723667
CGGCATA2450.038.2212871
CGCGGTG31400.037.61494410
GGGGTAG15200.037.4203571
GCCGGTT5950.036.5662963
CTACTCG32450.036.13620837
CGGTTCA6050.035.962995
TACTCGG32800.035.76265338
AGGATCG33600.034.31387759
TTAGCGG12800.033.99574310
CGAACTT6600.033.55257868
GATCGCT34950.033.4493761
CTTAGCG13200.033.2272039
CGGTATA1056.0936145E-1033.0307431
GTAGTCC36050.032.7246427
ACTCGGG37500.032.68109539
CGGGTAG4600.032.420391
GGGGTAC9850.032.3936041
Produced by FastQC (version 0.11.5)