EGAF00005318494 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005318494
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3434190
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG	16162	0.4706204374248367	No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC	12251	0.35673623183341635	No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC	9483	0.27613498379530543	No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG	9224	0.2685931762657279	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	6941	0.20211461800308078	No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG	6749	0.19652377998887657	No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT	6691	0.19483488100541904	No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA	5685	0.16554121932682817	No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT	5208	0.15165148113528956	No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT	4798	0.13971271245912426	No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA	4664	0.13581077342837758	No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT	4350	0.12666742375931442	No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG	4116	0.11985358992950303	No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG	4098	0.11932944886567137	No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG	3961	0.11534015299095274	No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG	3721	0.10835160547319747	No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC	3622	0.10546882962212342	No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT	3520	0.10249869692707742	No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG	3476	0.10121746321548894	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGCGGCA	4790	38.051662	32
GCGGCAC	4890	37.215748	33
CGGCACC	5085	35.528954	34
TCGCCGG	4830	35.51151	48
CCCGTCG	4885	35.31366	44
CCGTCGC	4880	35.22832	45
CCCCCGA	5525	33.277298	19
ACCCGCG	5545	32.897144	25
GTTCCGT	285	32.747623	1
GCACCCC	5720	31.908466	36
GGCACCC	5785	31.36079	35
CCCCGAC	5955	30.825478	20
CACCCCC	5885	30.741152	37
CCCCCGT	5940	29.072844	42
GATAGGC	3600	28.880907	40
CCGCGCG	6350	28.855362	27
ATAGGCA	3670	28.25318	41
CGTCGCC	6145	27.989492	46
CCCGCGC	6595	27.982445	26
GCATTAG	3725	27.937666	12

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content