FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3443784
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC150630.43739677052916215No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA126030.3659637189788907No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC123730.3592850190371986No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG87590.25434231647513317No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN70710.20532646646828023No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT63040.18305445405402893No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC42830.12436900804463925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC18300.058.5559127
GTCTACG18400.058.0504154
TCTACGG18550.057.581015
CTACGGC19450.055.0937426
GCGCGGT65100.053.244959
CGCGGTG69100.050.25955610
TGGCGCG69500.049.8733315
GCCGGTT9900.048.7252733
CGGTCTA2500.048.2436372
CCGAACT9900.048.11951467
GCCATAC22350.047.47985511
ACGGCCA22650.047.3100788
CGAACTT10300.046.3447468
CGGCCAT23050.046.040639
CGCGTGC79000.044.24477418
GGCGCGT79150.044.05393616
CGTGCCT79650.043.86533420
GGGTCTA19200.043.6131132
GCGTGCC80200.043.51199719
CTACTCG80850.043.30249437