FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3545095
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC150560.42469947913948713No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA129930.36650639827705606No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC124540.35130229232220855No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG88970.25096647621572904No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN72780.20529774237361764No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT64590.18219539955910913No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC42260.11920696060331246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG19300.061.5948834
TCTACGG19750.060.365925
TACGGCC19900.059.5738567
CTACGGC19950.059.417836
GCGCGGT68450.053.066149
CGGCCAT23250.050.9900979
TGGCGCG71900.050.79882415
GCCATAC23300.050.58414511
CCGAACT10500.050.40767767
CGCGGTG72450.050.37417210
ACGGCCA23800.050.2461628
GCCGGTT10850.047.9538963
CCATACC24750.047.48138812
GGGTCTA19400.047.4233672
GAACGCG24700.047.13733724
CGGTTCA10900.047.1023525
CATACCA25000.046.86673413
CGAACTT11850.046.34485268
GCGTCTA5250.045.9432332
TGAACGC25600.045.3349323