FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3509452
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC147280.4196666602079185No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA128080.3649572639830948No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC124930.3559815036649597No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG88410.25191967292899287No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN72100.20544518061509318No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT63110.1798286456119075No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC41920.11944884842419842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG18600.060.7609834
TCTACGG19300.058.5622335
TACGGCC19550.057.637927
CTACGGC21000.053.6581576
GCGCGGT66550.053.2272159
TGGCGCG70050.050.66468415
CCGAACT11500.050.05889567
CGCGGTG71750.049.32160610
GCCATAC22800.049.26873811
CGGCCAT23500.048.2410479
GCCGGTT12200.048.0123523
CCATACC23500.047.94573612
ACGGCCA23850.047.6796268
CGGTTCA12250.047.5391855
GGGTCTA19350.047.365772
CATACCA24500.046.12874613
GAACGCG24100.046.00787724
TGAACGC24400.045.56811523
CGTGCCT78550.045.45734820
CCGGTTC12900.045.139934