FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3470520
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN170210.4904452358724341No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA158480.45664626626557403No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC115650.33323536530548736No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC94140.27125618062999207No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT51890.1495164989684543No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC46170.1330348189896615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT53600.053.672639
CCGAACT8750.050.35989467
GTCTACG6250.049.3762554
TGGCGCG58000.049.3651515
CGGTTCA9050.048.1183665
GCCGGTT9250.047.0779653
TCTACGG6750.045.2107735
CGCGGTG64150.045.1651210
TACGGCC7150.043.1610647
GGGGACT41800.043.1098181
CTACTCG66650.043.00610437
GTAGTCC67400.042.91193427
TACTCGG67200.042.78639638
TGTAGTC67700.042.69378726
TAGTCCC68100.042.4479928
CGTGCCT67350.042.30505820
ACTCGGG70600.041.59294539
CCGGTTC10550.041.2768864
GCGTGCC69500.041.27029819
GGACTCT44050.041.255863