FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3519476
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN167880.4770028265571352No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA161610.4591876745288219No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC113130.32143989616636115No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC94780.2693014528299099No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT49560.14081641698934727No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC46740.1328038605746992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT56000.050.70569
CCGAACT8950.050.04670767
GCCGGTT8950.049.427413
TGGCGCG57250.049.41807615
GGGGACT43650.046.377721
CGCGGTG62700.045.232610
GGACTCT43850.044.2637333
TCTGGTC41650.043.88598310
ACTCTTC43950.043.3839955
CGTGCCT66450.043.08191320
CGGTTCA10150.042.9092865
GTCTACG5200.042.865834
CGAACTT11000.042.68334268
CTACTCG67900.042.48875837
GGGACTC46500.042.4049072
TACTCGG68250.042.20390338
TCTACGG5050.042.103085
GTAGTCC68450.041.78014827
TAGTCCC68500.041.7778628
TGTAGTC68550.041.70002726