FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4001648
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG194310.48557494312343313No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACGACTTTCTGATAGGCAGCC130240.3254659080458851No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG123570.30879777531656954No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC111520.2786851817051375No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG97100.24265002818838638No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC86560.21631087991747397No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT80970.20234163524627852No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT67100.16768091546282932No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA63330.15825979696365097No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT62180.15538598097583795No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG62030.15501113541221018No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG58700.14668956389967333No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG55940.13979240552892208No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT53700.13419471177874717No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG52840.13204559721394787No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG52640.1315458031291108No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG50990.12742250192920515No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG48470.12112509646025836No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG47960.1198506215439239No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG46280.11565235123129271No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT46270.11562736152705086No Hit
GGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACT45050.11257861760954486No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44830.11202884411622412No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA44430.11102925594655001No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA43040.10755568705693255No Hit
TGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAG42900.10720583119754662No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC42700.10670603711270957No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACGACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCC42610.10648112977453289No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC42480.10615626361938882No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG41410.1034823652655106No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG41290.10318248881460838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT37300.055.2143370
GTTCCGT4050.039.2476431
ATGGCCG44150.036.92585469
CGCGGCA56500.036.9151832
GCGGCAC58700.035.6044333
TCAGGAT23150.035.1917770
CGGCACC59600.035.07868634
CCGTCGC58250.033.87140745
TCGCCGG59400.033.34544848
CCCGTCG60150.033.12724744
GCACCCC65400.032.24979836
CTTACCC18550.031.91597770
TTATCGG3150.031.707887
GGCACCC67150.031.14636435
CACCCCC67850.031.04947537
GTCCGTT3450.030.9492072
CCCCCGA69300.030.73312419
TATGGCC50250.030.48864268
TATCGGA3300.030.2666138
TACGGGT18200.030.0888446
Produced by FastQC (version 0.11.5)