FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4001648
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG481461.2031543004282237No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC255200.6377372522520721No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC224850.5618934998780503No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA208490.5210103437383798No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG140860.35200497395073227No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT121320.3031750918621528No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG84330.21073817587154092No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG59910.14971331811293745No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC54940.13729343510473685No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC54840.13704353806231834No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC48960.12234959196810916No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC48870.12212468462993248No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC48070.12012550829058428No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44650.11157902943987076No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC41670.10413209757579878No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC41490.10368228289944542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG96050.065.098674
TCTACGG96100.064.921485
CTACGGC97500.063.9186446
TACGGCC96700.063.7353177
CGTCTAC21250.062.0659183
GCGTCTA18200.061.686792
GGGTCTA71550.061.60932
ACGGCCA100350.061.5200278
CGGCCAT102300.060.1820839
GCCATAC102100.060.03167311
GGCCATA104550.059.05232210
CATACCA104700.058.80696513
CCATACC105600.058.30576712
CCTGGTT154650.057.4647970
CGGTCTA11000.057.2933652
TACCACC110250.056.4088315
CCGAACT19450.055.44681567
GCGCGGT114850.054.9249659
ATACCAC114500.054.4353514
ACCACCC116950.053.20464716