FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4140869
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG500321.208248799949962No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC272430.6579053816964507No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC235960.5698320811404562No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA225240.5439437953724207No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG143150.34570038318043866No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT127020.3067472069268552No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG89440.21599330961689442No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG61270.14796411091488285No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC58970.14240972124450205No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC58930.14231312316327804No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC51230.12371799252765543No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC50450.12183432994378716No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC49600.11978162071777686No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47040.11359934351944001No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC43750.10565415133876488No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG42690.10309430218632852No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA42580.10282865746296249No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC42090.10164533096796832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG95750.065.142864
TCTACGG96250.064.840265
TACGGCC97050.064.071157
CTACGGC97550.063.8379486
GCGTCTA18400.061.617652
ACGGCCA101450.061.5641258
GGGTCTA70150.061.553172
CGGCCAT102250.060.9139029
CGTCTAC21750.059.8907283
GCCATAC103350.059.73194511
CCATACC105150.058.77570312
CATACCA105550.058.584213
GGCCATA106400.058.5056310
TACCACC109400.056.35407315
CGGTCTA12800.056.268242
CCTGGTT156550.056.25870570
ATACCAC112500.055.07822814
GCGCGGT122600.054.739029
CCGAACT18900.054.32951467
ACCACCC115000.053.6105216
Produced by FastQC (version 0.11.5)