FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4085331
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG480891.1771139229599756No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC266160.6515016775874464No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC233550.571679504059769No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA221210.541473873230835No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG138480.338968862008978No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT127100.311113101973867No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG86930.21278569594483285No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG57720.14128598147861215No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC55300.13536234885251647No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC54590.13362442357791818No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC50920.12464106335569872No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC50530.1236864283457081No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC49970.12231567038264463No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46310.11335678798119418No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC42940.10510776238204445No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA41920.10261102466360741No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC41840.1024152020974555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG95200.064.650544
TCTACGG95350.064.441215
TACGGCC96400.063.4890637
CTACGGC96950.063.1644366
GGGTCTA69700.061.6540152
ACGGCCA99450.061.4726338
GCGTCTA18550.060.5621572
CGGCCAT101150.060.506889
GCCATAC101300.059.87298211
CGTCTAC22200.059.2977333
GGCCATA103500.058.8999810
CCATACC103150.058.69893612
CATACCA104350.058.12298213
CCTGGTT148900.057.5002470
CCGAACT18250.056.0870367
TACCACC109650.055.43928515
CGGTCTA11750.055.1375732
GCGCGGT120700.054.9891139
ATACCAC111100.054.62267714
CGGTTCA18850.053.383195
Produced by FastQC (version 0.11.5)