FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4732179
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG241110.5095115801832517No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG101140.21372817892138063No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACGACTTTCTGATAGGCAGCC97800.20667011962142598No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC88350.18670046082365016No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG82250.17380999323990068No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN65860.13917478607635086No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC65760.13896346693563366No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG63980.13520198623086743No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC60400.12763676099319152No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT59620.1259884716955973No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA56660.11973342513036805No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT50110.10589202141339116No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA48930.10339845555292816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT27500.044.8478870
CGCGGCA73650.036.97918332
AGGACCG6650.036.3352570
GCGGCAC76350.035.7248333
CGGCACC79150.034.42529734
TCGCCGG75100.033.54045548
CCCGTCG75550.033.26663244
GTTCCGT4800.033.1611141
ATGGCCG30000.033.07343769
CCGTCGC75950.033.01386345
ACCCGCG85850.031.93132825
GCACCCC86850.031.50969736
CCCCCGA87700.031.40754319
CACCCCC89400.030.6660437
GGCACCC90950.029.89316235
TATCGGA2200.029.7938868
ACGACTT30250.029.76060530
GTCGGTT4200.029.5698724
CGACTTT30700.029.33403631
CACGACT31150.028.88939729