FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4732179
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG244760.5172247288194297No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC186790.39472302294566625No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC165950.35068411402020083No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA126560.267445504491694No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT88130.18623555871407232No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG73690.15572107479450797No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC72940.15413618123912895No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN65790.13902686267784883No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG47710.10082036203617827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC54650.063.4828957
TCTACGG55650.063.272365
GTCTACG56050.062.882354
CTACGGC56950.061.585826
ACGGCCA60100.057.840898
CGGCCAT61350.056.4949469
GCCATAC61650.056.05339411
GGGTCTA44950.055.1776242
GCGCGGT94400.054.0324069
CATACCA64500.053.95147713
CGTCTAC14850.053.424153
CCATACC65100.053.2952912
GCGTCTA12500.052.7092862
GGCCATA66600.052.09440210
CCGAACT15800.051.13616667
GCCGGTT16000.050.8777923
TACCACC69550.050.57954415
CGCGGTG103300.049.4449610
CGGTCTA7600.049.0200732
TGGCGCG104050.048.9896415