FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4743363
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG250820.5287809514051528No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC192730.40631509753733797No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC171360.3612626737612112No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA129720.27347685597749954No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT91050.19195241856885084No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC76300.16085633758158505No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG75150.15843189736901858No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN65500.138087681672265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG53700.064.211314
TCTACGG54450.063.2635275
TACGGCC55400.062.1164447
CTACGGC56350.061.1304176
GCGTCTA11350.058.6435972
ACGGCCA60450.057.2124338
GGGTCTA42500.056.2345852
GCCATAC61000.055.79097711
CGGCCAT61800.055.6836749
CGTCTAC13700.055.1205983
CATACCA63400.053.73340213
CCATACC63750.053.38430412
GGCCATA64500.053.24468210
GCGCGGT97500.052.5533039
CCGAACT16800.050.88868767
TACCACC69100.049.15231715
CGGTTCA17050.048.739215
TGGCGCG105100.048.72025315
ATACCAC70950.048.55005314
GCCGGTT17600.048.1962053