FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4881127
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG267600.5482340451293318No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG109320.22396467045417995No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACGACTTTCTGATAGGCAGCC98660.2021254517655451No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC91590.18764109190357062No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG87600.179466750199288No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG72640.14881809057621323No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC68360.14004962378565441No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN67250.13777555880025247No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC63830.13076898019658165No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA62630.1283105315637147No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT62180.1273886133263896No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT52820.1082127139900273No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA51930.10638936458731764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT25200.040.3038370
CGCGGCA82150.037.44907832
GCGGCAC84400.036.5467633
CGGCACC86700.035.59044634
GTTCCGT4700.034.5346571
TCGCCGG82650.034.49878748
CCCGTCG82800.034.35000244
CCGTCGC84100.033.79583445
GCACCCC96050.032.5855936
CCCCCGA95800.032.52591719
ACCCGCG96800.032.17408825
CACCCCC98500.031.71870837
TAGTCCG5100.031.106416
GGCACCC99700.031.03371635
ATGGCCG27400.030.05477569
TTCCGTT7000.029.5646932
CCCCGAC105950.029.41718520
GTCGGTT4000.029.3183214
ACGACTT31650.029.3163930
CGACTTT32950.028.37807831