FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4881127
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG250870.5139591737727783No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC194830.39914962261789133No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC178710.3661244626497118No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA134990.2765549841255923No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT93000.1905297690471893No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC77050.1578528893019993No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG74980.1536120654103038No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN67310.13789848123189585No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG49760.10194366997621657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG49550.063.111034
TCTACGG49800.062.7249765
TACGGCC50050.062.0825087
CTACGGC51100.060.932446
ACGGCCA54250.057.657548
CGGCCAT55500.056.172549
GCCATAC56400.055.45960211
CCATACC58150.053.9684912
GGGTCTA44350.053.642682
CATACCA58750.053.59012213
GCGCGGT99150.053.4600949
GCGTCTA8750.053.1962132
GGCCATA60750.051.48842210
CCGAACT15900.050.38340867
TACCACC63000.049.85623615
TGGCGCG107250.049.79615415
GCCGGTT16100.049.6825373
CGCGGTG107650.049.62333710
CGGTTCA16450.049.0446475
ATACCAC66800.047.3312414