FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4825832
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG257150.5328614837814495No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG108130.2240649902441693No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACGACTTTCTGATAGGCAGCC98140.2033638966296382No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC89950.18639272979250002No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG83020.17203251169953698No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC68390.1417164957255039No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG68160.1412398939706148No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN63850.13230879152030156No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC62310.12911763194408757No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT61290.12700400677023155No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA59650.1236056290397179No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA51640.10700745488031908No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT51510.10673807127972959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT26300.044.29383570
CGCGGCA77700.037.81347332
GCGGCAC80450.036.7038833
CGGCACC83150.035.43940434
GTTCCGT5400.035.1999971
TCGCCGG79400.034.453448
CCCGTCG79000.034.42671644
CCGTCGC79500.034.39657245
GCACCCC90000.032.9222336
ACCCGCG90750.032.3709225
CCCCCGA91750.032.2778519
CACCCCC92550.032.03151337
ATGGCCG29150.031.49565769
GGCACCC94800.031.17070635
TAGTCCG4450.030.2329276
CCCCGAC99650.029.69078320
AGGACCG6900.029.23465270
CCAGCGT26800.027.4729470
AGTCCGC107850.027.411727
CGTCGCC100900.027.25344846