FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4825832
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG248140.5141911280790545No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC193100.4001382559525487No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC173920.36039381395788334No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA135780.2813608099080117No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT92670.19202906358944943No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC76410.1583353917003327No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG72600.15044038002151752No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN63770.13214301699686187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG52100.063.2160154
TCTACGG52150.063.091245
TACGGCC52750.062.3736087
CTACGGC54400.060.7353556
ACGGCCA57500.057.1010448
GCGTCTA11300.056.156612
GCCATAC58350.055.9731311
CGGCCAT58700.055.933159
GCGCGGT97750.054.2284939
CCATACC60500.053.86887712
GGCCATA62300.052.86713810
CATACCA61650.052.86401713
GGGTCTA44950.052.632732
CGTCTAC13700.052.1088183
TACCACC64300.050.739515
TGGCGCG105200.049.9942515
CGCGGTG106950.049.62817410
CCGAACT15150.048.6339467
CGGTTCA15550.048.3505135
CGGTCTA7300.047.7155532