FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3521432
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG78080.22172797884496986No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG36810.10453133838733789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT14700.035.76883770
CGCGGCA24650.033.4549832
TCGGATA5200.032.66426534
GACGTAT5350.031.84235844
TCGCCGG26200.030.30909248
CCGTCGC25950.030.29016145
GCGGCAC27550.030.19268233
CCCGTCG26800.029.57760244
CGGATAG5750.029.5461735
ACGTATC5850.029.13202945
GGACGTA5750.029.0147243
CGGCACC29200.028.37234334
ATGGCCG14600.026.72950269
CGCCCTA7900.026.69613561
CGTATCA6050.026.45683746
GTGCCGT5100.025.8266941
AGTCCGC33750.024.7848287
CCCCCGA33950.024.5415419
CGTACGT1851.8189894E-1124.2938419
TAGTCCG2001.8189894E-1224.1959326
Produced by FastQC (version 0.11.5)