FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3521432
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG110870.31484350684607854No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC70700.20077059559860874No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC53890.15303433375967504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG23050.060.5627484
TCTACGG23450.059.3823435
TACGGCC23350.058.8959127
CTACGGC24250.057.4233366
ACGGCCA26300.052.8152438
CGGCCAT27200.050.9406439
GCGCGGT35950.048.922419
GCCATAC28200.048.89195611
TGGCGCG37050.047.47262215
GAACGCG29400.046.8207424
GGGTCTA22350.045.9268462
CCATACC30250.045.46438612
ATCTCGT30450.045.19032735
CCTGGTT45050.044.97017370
GGTCTAC25300.044.9392743
TGAACGC30650.044.9086923
CTGAACG31100.044.5874322
CCCGATC31150.044.24510231
GCCCGAT31150.044.23439430
CATACCA31300.044.16002313
Produced by FastQC (version 0.11.5)