FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3503792
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG82730.23611561416887758No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG39120.11165046326950916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT15200.039.11219870
CGCGGCA26650.032.50135432
ATGGCCG14000.032.1476269
GCGGCAC28800.030.32475533
TCGCCGG27250.029.91504948
CCCGTCG28200.028.72295644
CCGTCGC28500.028.1904245
CGGCACC31400.027.8209634
ACCCGCG33800.025.9988625
GACGTAT4650.025.41840244
AGGACCG5800.025.17566770
AGTCCGC35800.025.0006567
TCGGATA5000.024.95937534
CAGTCCG34600.024.8696886
CGCGGTT1401.3504177E-824.7099571
CGGATAG5100.024.47523535
CCCCCGA36350.024.15394219
GCACCCC37250.024.11183236
CCCCGAC36450.024.09111620
AAACCGC6000.023.61387411
Produced by FastQC (version 0.11.5)