FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3503792
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG111060.31697087041696537No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC71660.2045212729522757No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC56740.16193883655194144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG24050.062.2077184
TCTACGG24500.060.92415
TACGGCC25250.059.2513127
CTACGGC25850.057.8760386
ACGGCCA28850.051.8577358
CGGCCAT29050.051.500719
CCGAACT6900.051.41671867
GCCATAC29250.051.26376711
GCGCGGT33450.049.5811469
GAACGCG30950.048.61029424
GGTCTAC26100.048.452733
GGGTCTA24800.048.2081762
CCATACC31500.047.6020712
CCTGGTT46950.047.4525370
TGAACGC31600.047.38404523
TGGCGCG35450.046.1984515
ATCTCGT32250.045.90442335
CCCGATC32450.045.46839531
CATACCA33050.045.2650613
CGTCTGA33050.045.2595839
Produced by FastQC (version 0.11.5)